Plasmid_Backbone

Part:BBa_M36095

Designed by: Joseph Shih   Group: Stanford BIOE44 - S11   (2013-04-23)


S. elongatus cyanobacteria sensor test rig

S. elongatus test vector for sensors. Has a cyanocitrus YFP coding sequence as a reporter. Comes with high copy number origin and kanamycin resistance

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 1509
    Illegal PstI site found at 1119
    Illegal PstI site found at 3078
    Illegal PstI site found at 4318
    Illegal PstI site found at 4651
    Illegal PstI site found at 8296
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 2489
    Illegal SpeI site found at 1509
    Illegal PstI site found at 1119
    Illegal PstI site found at 3078
    Illegal PstI site found at 4318
    Illegal PstI site found at 4651
    Illegal PstI site found at 8296
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 2505
    Illegal BamHI site found at 2515
    Illegal BamHI site found at 5060
    Illegal XhoI site found at 3234
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 1509
    Illegal PstI site found at 1119
    Illegal PstI site found at 3078
    Illegal PstI site found at 4318
    Illegal PstI site found at 4651
    Illegal PstI site found at 8296
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 1509
    Illegal PstI site found at 1119
    Illegal PstI site found at 3078
    Illegal PstI site found at 4318
    Illegal PstI site found at 4651
    Illegal PstI site found at 8296
    Illegal NgoMIV site found at 5086
    Illegal NgoMIV site found at 5458
    Illegal NgoMIV site found at 5618
    Illegal NgoMIV site found at 5972
    Illegal AgeI site found at 1913
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2776
    Illegal BsaI.rc site found at 8122
    Illegal SapI site found at 7039


[edit]
Categories
//plasmidbackbone
Parameters
None